Human IL-2 ELISA Kit說明書

【資料簡介】

• Product name

  EliKine™ Human IL-2 ELISA Kit

• Reactivity

  Human

• Applications

  ELISA

• Application notes

  EliKine™ Human IL-2 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-2 in samples. An antibody specific for IL-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-2 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Detection method

  Colorimetric

• Sample type

  Cell culture supernatants, Other biological fluids, Plasma, Serum

• Assay type

  Sandwich ELISA (quantitative)

• Assay duration

  Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Fig.1. Human IL-2 Standard Curve.

Fig.2. EliKine™ Human IL-2 ELISA Kit is a sandwich ELISA to quantitate IL2 in samples.

Product Properties

• Kit components

  • Human IL-2 microplate
  • Human IL-2 standard
  • Human IL-2 detect antibody
  • EliKine™ Streptavidin-HRP
  • Standard diluent
  • Assay buffer
  • HRP substrate
  • Stop solution
  • Wash buffer
  • Plate covers.

• Features & Benefits

  EliKine™ Human IL-2 ELISA Kit has high sensitivity and excellent specificity for detection of Human IL-2. No significant cross-reactivity or interference between Human IL-2 and analogues was observed.

• Calibration range

  3.9 pg/ml-250 pg/ml

• Limit of detection

  2 pg/ml

• Usage notes

  • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
  • Allow all reagents to warm to room temperature for at least 30 minutes before opening.
  • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
  • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
  • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
  • It is recommended that all samples and standards be assayed in duplicate or triplicate.

• Storage instructions

  The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

• Shipping

  Gel pack with blue ice.

• Precautions

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

• Background

  The protein encoded by IL-2 gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of IL-2 gene in the immune response to antigenic stimuli

• Gene ID

  3558

• Alternative names

  IL 2; IL2; IL-2; TCGF; lymphokine; T cell growth factor; aldesleukin; interleukin-2; involved in regulation of T-cell clonal expansion

• Accession

  P60568