熒光素標記*酶相關蛋白2抗體IgG相關知識

Agarose plug: 
1% agarose dissolved in 1x Resolving gel buffer. 
（I make 50 ml, keep melting it as I need it, and re-adding water to maintain agarose conc.） 
Resolving gel: 24 ml of a 9% gel 
5.4 ml 40% acrylamide/bisacrylamide （29:1 mix） 
3 ml 8x Resolving gel buffer 
15.6 ml water 
12 µl TEMED 
60 µl 20% ammonium persulfate 
Stacking gel: 8 ml 
1 ml 40% acrylamide/bisacrylamide （29:1 mix） 
2 ml 4x Stacking gel buffer 
5 ml water 
8 µl TEMED 
21.6 µl 20% ammonium persulfate 

After flash spinning the samples, load into the wells. 
Be sure to use markers. 
We use 15 µl Bio-Rad Kaleidoscope Prestained Standards #161-0324 directly. 
Run with constant current （35 - 37 mA with voltage set at > 300 V）. 
Usual running time is about 2.5 hr. 

After flash spinning the samples, load into the wells. 
Be sure to use markers. 
We use 15 µl Bio-Rad Kaleidoscope Prestained Standards #161-0324 directly. 
Run with constant current （35 - 37 mA with voltage set at > 300 V）. 
Usual running time is about 2.5 hr.