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武漢紐斯特生物科技有限公司
武漢紐斯特生物科技有限公司
Anti RHEB(Y35N) Mouse Monoclonal Antibody
RHEB(Y35N) [26338]
[100ul]
| Catalog Number: 26338 |
| Gene Symbol: RHEB |
| Description: Anti RHEB(Y35N) Mouse Monoclonal Antibody |
| Background: RheB(Ras Homolog Enriched in Brain) is an evolutionarily conserved member of the Ras-family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, RheB is widely expressed in other tissues and may be induced by growth factor stimulation. RheB has an important role in regulating the insulin/TOR signaling pathway. The mammalian target of rapamycin, mTOR, is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with translation and cell growth. The tuberin/hamartin (TSC2/TSC1) complex inhibits mTOR activity indirectly by inhibiting RheB via tuberin's GAP activity. |
| Immunogen: A synthetic peptide from the internal region of RHEB which includes the mutation of Y35N, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes Y35N mutant, but not wild-type RHEB of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Download Datasheet: |
| Western blot: |
| Western blot analysis of recombinant RHEB(Y35N) and wildtype proteins. Purified His-tagged RHEB(Y35N) protein (lane2) and corresponding wild-type protein (lane1) were blotted with Anti RHEB(Y35N) monoclonal antibody (Cat. #26338). |
| Immunofluorescence: |
| Immunofluorescence of cells expressing RHEB proteins with Anti RHEB(Y35N) antibody. HEK293T cells were transfected with pCDNA3-GFP RHEB(WT) plasmid (left column) or pCDNA3-GFP RHEB(Y35N) plasmid (right column), then fixed and stained with Anti RHEB(Y35N) monoclonal antibody (Cat. #26338). |
| Related Products | |||
| Catalog# | Name | Size | Price |
| Active RheB GTP Monoclonal Antibody | 30 μL | ￥4800 | |
| RheB Activation Assay Kit | 20 assays | ￥6800 | |
| Anti-RheB Mouse Monoclonal Antibody | 100 μL | ￥2800 | |
| Anti-RheB Rabbit Polyclonal Antibody | 100 μL | ￥2600 | |
| Publications: |
| 1. Cancer Res. 2014 Feb 15;74(4):1067-78 |
| 2. PLoS One. 2013 Dec 31;8(12):e83941 |
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