FAM tetrazine,FITC-tetrazine
Tetrazine (methyltetrazine) derivative of fluorescein, pure 5-isomer.
Tetrazines are reactive heterodiene components for [4+2] inverse electron demand Diels-Alder reaction (ied-DA) with strained cycloalkenes (trans-cyclooctenes, cyclopropenes). This reaction is also called TCO (tetrazine-cyclooctene) ligation. The reaction is among the fastest bioconjugation reactions.
General properties
Appearance: | yellow solid |
Mass spec M+ increment: | 529.12 |
Molecular weight: | 559.53 |
Molecular formula: | C31H21N5O6 |
Solubility: | good in DMF, DMSO |
Quality contol: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Spectral properties
Excitation maximum, nm: | 490 |
ε, L⋅mol−1⋅cm−1: | 80000 |
Emission maximum, nm: | 513 |
Fluorescence quantum yield: | 0.93 |
CF260: | 0.20 |
CF280: | 0.17 |
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疊氮化物 Dye azides
Pyrene azide 1/ Pyrene azide 2
PEP azide
Perylene azide
BDP FL azide
Coumarin 343 azide
FAM azide, 5-isomer
FAM azide, 6-isomer
TAMRA azide, 5- isomer
BDP TMR azide
Cyanine3 azide
Cyanine3.5 azide
Cyanine5 azide
Cyanine5.5 azide
Cyanine7 azide
Cyanine7.5 azide
Sulfo-Cyanine3 azide
Sulfo-Cyanine5 azide
Sulfo-Cyanine7 azide
