常用抗體標記熒光染料的特性及其應用
供應商:西安凱新生物科技有限公司
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1、FITC:激發波長488nm,zui大發射波長525nm。
1)其標記的抗體適用于所有配備488nm氬離子激光器的流式細胞儀;
2)在流式細胞儀的FL1通道檢測;
3)可用于熒光顯微鏡技術
4)熒光強度易受PH值影響,PH值降低時其熒光強度減弱。
2、Alexa Fluor 488:激發波長488nm,zui大發射波長519nm。
1)其標記的抗體適用于所有配備488nm氬離子激光器的流式細胞儀;
2)在流式細胞儀的FL1通道檢測;
3)具有超乎尋常的光穩定性,非常適用于熒光顯微鏡技術;
4)在較寬的PH值范圍內保持穩定(PH4~10)。
3、Cy3:激發波長488nm,zui大發射波長570nm。
1)其標記的抗體適用于所有配備488nm氬離子激光器的流式細胞儀;
2)在流式細胞儀的FL2通道檢測;
3)適用于熒光顯微鏡技術;
4)為小分子染料,非常適合需小分子染料的流式細胞術,熒光強度低于PE。
4、Cy5:激發波長633/635nm,zui大發射波長670nm。
1)其標記的抗體適用于所有配備633nm氬離子激光器的流式細胞儀;
2)在流式細胞儀的FL4通道檢測;
3)適用于熒光顯微鏡技術;
4)同樣為小分子染料,非常適合需小分子染料的流式細胞術,熒光強度低于APC。
5)與單核和粒細胞非特異性結合多,易出現假陽性結果。
5、PE:激發波長488nm,zui大發射波長575nm。
1)其標記的抗體適用于所有配備488nm氬離子激光器的流式細胞儀;
2)在流式細胞儀的FL2通道檢測;
3)其熒光泯滅性強,不適用于傳統的熒光顯微鏡技術,但適用于激光共聚焦顯微鏡技術。
6、PE-TR:激發波長488nm,zui大發射波長615nm。
1)在Beckman Coulter流式細胞儀的FL3通道檢測;
2)可適用于小功率激光器的流式細胞儀,也可使用于大功率激光器的大流式細胞儀。
7、PE-Alexa Fluor 610:激發波長488nm,zui大發射波長628nm。
1)在Beckman Coulter流式細胞儀的FL3通道檢測;
2)熒光強度高;
3)可適用于小功率激光器的流式細胞儀,也可使用于大功率激光器的大流式細胞儀。
官能團目錄
炔烴 Dye alkynes
1-Ethynyl pyrene
3-Ethynyl perylene
BDP FL alkyne
FAM alkyne, 5-isomer
FAM alkyne, 6-isomer
TAMRA alkyne, 5-isomer
BDP TMR alkyne
Cyanine3 alkyne
Cyanine5 alkyne
Cyanine5.5 alkyne
Cyanine7 alkyne
Cyanine7.5 alkyne
Sulfo-Cyanine3 alkyne
Sulfo-Cyanine5 alkyne
疊氮化物 Dye azides
Pyrene azide 1/ Pyrene azide 2
PEP azide
Perylene azide
BDP FL azide
Coumarin 343 azide
FAM azide, 5-isomer
FAM azide, 6-isomer
TAMRA azide, 5- isomer
BDP TMR azide
Cyanine3 azide
Cyanine3.5 azide
Cyanine5 azide
Cyanine5.5 azide
Cyanine7 azide
Cyanine7.5 azide
Sulfo-Cyanine3 azide
Sulfo-Cyanine5 azide
Sulfo-Cyanine7 azide
N-羥基琥珀酰亞胺脂Dye NHS esters
Pyrenebutyric acid NHS ester
ROX NHS ester, pure 6- isomer
BDP FL NHS ester
FAM NHS ester
Cyanine3 NHS ester
Cyanine3.5 NHS ester
Cyanine5 NHS ester
Cyanine5.5 NHS ester
Cyanine7 NHS ester
Cyanine7.5 NHS ester
Sulfo-Cyanine3 NHS ester
Sulfo-Cyanine5 NHS ester
sulfo-Cyanine5 bis-NHS ester
Sulfo-Cyanine7 NHS ester
馬來酰亞胺 Dye maleimides
Pyrene maleimide
BDP FL maleimide
Cyanine3 maleimide
Cyanine5 maleimide
Cyanine5.5 maleimide
Cyanine7 maleimide
Cyanine7.5 maleimide
Sulfo-Cyanine3 maleimide
Sulfo-Cyanine5 maleimide
伯胺 Primary amine
BDP FL amine
Cyanine3 amine
Cyanine5 amine
Cyanine5.5 amine
Cyanine7 amine
Cyanine7.5 amine
Sulfo-Cyanine5 amine
Sulfo-Cyanine7 amine
酰肼 Dye hydrazides
Pyrene hydrazide
BDP FL hydrazide
Cyanine3 hydrazide
Cyanine5 Boc-hydrazide
Cyanine5 hydrazide
Cyanine5.5 hydrazide
Cyanine7 hydrazide
Cyanine7.5 hydrazide
馬來酰亞胺 Dye maleimides
BDP FL maleimide
Cyanine3 maleimide
Cyanine5 maleimide
Cyanine5.5 maleimide
Cyanine7 maleimide
Cyanine7.5 maleimide
Sulfo-Cyanine3 maleimide
Sulfo-Cyanine5 maleimide
羧酸 Carboxylic acids
Pyrenebutyric acid
BDP FL carboxylic acid
Cyanine3 carboxylic acid
Cyanine3.5 carboxylic acid
Cyanine5 carboxylic acid
Cyanine5.5 carboxylic acid
Cyanine7 carboxylic acid
Cyanine7.5 carboxylic acid
Sulfo-Cyanine3 carboxylic acid
Sulfo-Cyanine5 carboxylic acid
Sulfo-Cyanine7 carboxylic acid
點擊化學試劑
非熒光炔烴 Non-fluorescent alkynes
Pentynoic acid STP ester
Biotin alkyne
非熒光疊氮化物 Non-fluorescent azides
Azidobutyric acid NHS ester
Biotin azide
寡核苷酸分析
亞磷酰胺 Phosphoramidites
FAM Phosphoramidite, 6-Isomer
Alkyne Amidite, hydroxyprolinol
Alkyne Phosphoramidite, 5'-terminal
DMS(O)MT aminolink C6
Long trebler phosphoramidite
Pyrene phosphoramidite dU
PCR/qPCR PCR/qPCR
ROX reference dye for qPCR
SYBR Green I Gel Staining Solution, 10000x
SYBR Green I for Real Time PCR, 100x
CPG固相修飾 CPG solid supports
Alkyne CPG modifier 500
TFA-Amino modifier CPG 500
三磷酸酯修飾 Modified triphosphates
Ami1-ddUTP
Ami1-dUTP