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上海瑞齊生物科技有限公司

Rabbit Anti-c-fos

時間:2012-4-2閱讀:486
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Rabbit Anti-c-fos
 
Cat. Number: RS-0469R
Quantity size:  0.1ml (dilute with pH 7.4    0.01 M PBS or diluent of antibody)
 
Background: The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. Nuclear phosphoprotein which forms a tight but non- covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, c-fos and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. Subunit: Heterodimer with JUN. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB. Belongs to the bZIP family. Fos subfamily. Subcellular Location: Nucleus.
 
Specificity:      ·anti- c-fos is a rabbit monoclonal antibody unconjugated
                ·specific for c-fos of  mouse
                ·epitope mapping near the amino terminus of c-fos of  
                   mouse
                · use for western blotting, elisa, immunoprecipitation    
                   and immunohistochemistry
· Protein A affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt: 82kDa
 Rabbit Anti-c-fos
Application:     · Western blotting: 1:100-500
· Immunohistochemistry: 1:100-500
· ELISA: 1:500-1000
· Optimal working dilutions must be determined by the end user.
 
Storage:   Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.
 
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Rabbit Anti-c-fos

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