Mouse anti-Iba-1/Aif1
（corticotropin releasing hormone）
Quantity size : 0.2ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
Background: Allograft Inflammatory Factor-1 （AIF1）or ionized calcium-binding adaptor molecule 1 （Iba1） is expressed selectively in microglia/macrophages and is a Ca2+-binding peptide produced by activated monocytes and microglial cells. It has been suggested that AIF1 expression is associated with chronic inflammatory processes. AIF1 is expressed by activated monocytes and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts.
Specificity : · anti-Iba-1 is a mouse monoclonal antibody unconjugated
· specific for Iba-1 of human and rat
· epitope mapping near the amino terminus of Iba-1 of
Human ,rat origin
· human, rat cross reacted
· use for western blotting, elisa, immunoprecipitation
and immunohistochemistry
· affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt:16kDa
Application: · Western blotting 1:100-500
· Immunohistochemistry 1:100-500
· ELISA 1:500-1000
· Optimal working dilutions must be determined by the end user.
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human,therapeutic or diagnostic applications.
