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人轉錄因子AP-1 ELISA kit
產品類型:
ELISA Kit
AP-1, AP1, c-Jun, Jun activation domain binding protein|activator protein 1|enhancer-binding protein AP1|v-jun avian sarcoma virus 17 oncogene homolog|v-jun sarcoma virus 17 oncogene homolog
96T
Human
jun oncogene
JUN
Transcription/Transcription regulation
Transcription
47 pg/ml-3000 pg/ml
11.75 pg/ml
1-5h
50-100ul
450 nm
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AP-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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