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技術文章

Anti-VEGF抗體說明書

閱讀:693發布時間:2012-9-30

 

Rabbit Anti-VEGF

Catalog Number : BYK- 1665R     
Quantity size :   100ug  (0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide)
 
Background: This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms.
Specificity :   
        Anti- VEGF 1 is a rabbit polyclonal antibody unconjugated                
        specific for VEGF of Rb, hu, mo, rat         
        use for western blotting,elisa,immunoprecipitation and immunohistochemistry
        Protein G affinity chromatography purification, purity :>95%
        Isotype:  IgG
        mol wt:  13/56kDa
 
Application :  
        Western blotting 1:100-500                
         Immunohistochemistry 1:100-500                 
         ELISA 1:500-1000
         IP=1:20-100 
         IF=1:100-500                  
         Optimal working dilutions must be determined by the end user.
 
Storage: Store at -20 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4
 
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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